Journal: Scientific Reports
Article Title: Identification of ITPR1 gene as a novel target for hsa-miR-34b-5p in non-obstructive azoospermia: a Ca 2+ /apoptosis pathway cross-talk
doi: 10.1038/s41598-023-49155-5
Figure Lengend Snippet: Histological feature of the human testicular tissues. ( a–c ) H&E stained testis section from adult OA and NOA. ( a ) OA testicle (Control). ( b ) NOA testicle. ( c ) NC. Magnification: × 40. H&E Hematoxylin and eosin, NOA non-obstructive azoospermia, OA obstructive azoospermia, NC negative control. Arrows: Irregularity in NOA patients’ seminiferous. ( d–f ) TUNEL-stained sections of the human testicular tissue for the detection of apoptosis. ( d ) OA, control group. A few TUNEL-stained nuclei were observed in the OA (Arrowheads). ( e ) In the NOA, the increase in the apoptotic cells was observed compared to the OA group (Arrows). ( f ) NC negative control. Green and Red fluorescent stained nuclei indicate apoptotic and viable cells, respectively. Magnification, × 40. TUNEL deoxy-UTP-digoxigenin nick end labeling, NC negative control. ( g ) Quantification of staining intensity reveals 2.7-fold increases in apoptosis rate in NOA compared with OA (control group).
Article Snippet: Then, 5-μm-thick cross-sections were prepared for subsequent hematoxylin–eosin (H&E) staining and deoxy-UTP-digoxigenin nick end labeling (TUNEL) assay (Promega kit, USA).
Techniques: Staining, Control, Negative Control, TUNEL Assay, End Labeling